We are interested in understanding the structure/activity relationships of human proteasome, a large multicatalytic complex in charge of intracellular protein degradation, which is a therapeutic target for some cancers.
Structural Mass Spectrometry of Protein Assemblies
Often reduced to the analysis of the primary structure of proteins, Mass Spectrometry has evolved over the past 20 years to provide information on the secondary, tertiary and even quaternary structure of proteins. Structural Mass Spectrometry encompasses an extending range of methods, including Native MS and ion mobility-MS, cross-linking, Hydrogen-Deuterium Exchange and top-down proteomics, aiming at collecting as much structural information on a biomolecule as possible.
Characterization of Inflammatory and Immune Processes
Taking advantage of the great improvements in large-scale proteomic analysis of complex samples, we use state-of-the-art mass spectrometry techniques to study the molecular mechanisms of the inflammatory and immune responses in specific cellular systems, such as endothelial cells and different types of immune cells.
Major research axis:
- Proteomic analysis of the inflammatory response of endothelial cells
- Functional mechanisms of interleukin-33, a cytokine playing a major role in innate immunity and allergy
- Fine molecular characterization of the T Cell Receptor (TCR) pathway of T lymphocytes
- In-depth proteomic analysis of regulatory T cells (Tregs)
High-resolution mass spectrometry-based proteomics and label-free quantitative methods have progressed tremendously in recent years, and became increasingly popular as an attractive and powerful way to analyse differential protein expression in complex biological samples, particularly in the field of biomarker discovery.
Data management and proteomics tools development
Proteomics data analysis requires the use of dedicated computer hardware to acquire and store data and tools to validate, quantify and classify proteins. The Toulouse Proteomics Infrastructure has gained over the years an important expertise in bioinformatics with specialized staff to handle data storage, data processing, and data analysis and also to develop new pipelines for data analysis.
The need to respond in a controlled manner to requests for scientific services or collaborations, the need to consolidate the quality of the expertise carried out by managing the skills of the personnel concerned and the control of the entire instrumental park led, as early as 2005 , the Toulouse Proteomics Infrastructure towards the implementation of an ISO 9001-type Quality approach.