We are interested in understanding the structure/activity relationships of human proteasome, a large multicatalytic complex in charge of intracellular protein degradation, which is a therapeutic target for some cancers.
Proteomics represent a powerful tool for the understanding of complex biological mechanisms. Taking advantage of the great improvements in large-scale proteomic analysis of complex samples, we use state-of-the-art mass spectrometry techniques to bring new insights in the molecular mechanisms of the inflammatory and immune responses in specific cellular systems such as endothelial or different types of immune cells.
Structural Mass Spectrometry of Protein Assemblies
Often reduced to the analysis of the primary structure of proteins, Mass Spectrometry has evolved over the past 20 years to provide information on the secondary, tertiary and even quaternary structure of proteins. Structural Mass Spectrometry encompasses an extending range of methods, including Native MS and ion mobility-MS, cross-linking, Hydrogen-Deuterium Exchange and top-down proteomics, aiming at collecting as much structural information on a biomolecule as possible.
High-resolution mass spectrometry-based proteomics and label-free quantitative methods have progressed tremendously in recent years, and became increasingly popular as an attractive and powerful way to analyse differential protein expression in complex biological samples, particularly in the field of biomarker discovery.
Proteomics data analysis requires the use of dedicated computer hardware to acquire and store data and tools to validate, quantify and classify proteins. The Toulouse Proteomics Infrastructure has gained over the years an important expertise in bioinformatics with specialized staff to handle data storage, data processing, and data analysis and also to develop new pipelines for data analysis.
The need to respond in a controlled manner to requests for scientific services or collaborations, the need to consolidate the quality of the expertise carried out by managing the skills of the personnel concerned and the control of the entire instrumental park led, as early as 2005 , the Toulouse Proteomics Infrastructure towards the implementation of an ISO 9001-type Quality approach.